覃重军

1987年毕业于武汉大学生物系微生物专业，获学士学位。

1992年毕业于华中农业大学微生物系，获博士学位。 1994年被破格提拔为副教授。主持国家自然科学青年基金和湖北省自然科学基金各一项。

1994年，覃重军确立了自己的5个科学梦想：要在重大基础研究、原创技术、产业化、解决人类疾病、新理论上有所突破。

1995年至2000年在美国斯坦福大学基因工程创始人之一Stanley N. Cohen教授实验室作博士后, 从事链霉菌线型质粒的复制、重组、修复、 接合转移、端粒基因、异常DNA结构等方面的研究。

2001年通过“引进国外杰出人才计划”答辩, 被中国科学院上海植物生理研究所聘为研究员、博士生导师。

2001年回国后主持国家“863”项目和国家自然科学基金共4项，主持上海市、科学院、所基金共5项。

2002年任微生物次生代谢分子调控研究开放实验室主任。

2003年获国家杰出青年科学基金资助。

2013年5月8日，覃重军瞄准了真核单细胞生物的模式材料——酿酒酵母，花了5年时间，用基因编辑的方法，将酿酒酵母中16条天然染色体合成为1条，创建出了国际首例人造单染色体真核细胞，实现“人造生命”里程碑式的重大突破。该成果于2018年8月2日在线发表在《自然》杂志上。

分子微生物学和分子微生物组学

从事分子微生物学、分子微生物组学和微生物药物代谢工程的研究，主要内容包括：（1）放线菌线型染色体和线型质粒的生物学功能。（2）中草药植物内生放线菌。（3）放线菌基因组工程。（4）微生物药物工业生产菌种的遗传改良。

2019年6月28日，被评为科学中国人（2018）年度人物。

博士毕业论文发表在国际知名专业期刊《细菌学杂志》上，这也是华中农大在该本杂志上发表的第一篇学术文章。

两年前，覃重军带领团队将酿酒酵母中16条天然染色体合成为1条，创建出了国际首例人造单染色体真核细胞。这一成果在线发表在《自然》杂志上，被业界认为是合成生物学具有里程碑意义的重大突破。

带领团队首次在粘细菌中发现质粒并建立遗传操作系统，全世界有20多个实验室来函索取质粒载体;对大肠杆菌的基因组进行了“删减、重组、优化、合成”等操作，做了大量有益的探索。他的团队与国内制药企业合作改良了抗寄生虫药物多拉菌素，打破了国外在该领域的垄断，药品销售额超过亿元。

1. Yangyang Shao, Ning Lu, Zhenfang Wu, Chen Cai, Shanshan Wang, Ling-Li Zhang, Fan Zhou, Shijun Xiao, Lin Liu, Xiaofei Zeng, Huajun Zheng, Chen Yang, Zhihu Zhao, Guoping Zhao*, Jinqiu Zhou*, Xiaoli Xue* and Zhongjun Qin* (2018) Creating a functional single chromosome yeast. Nature (article). 560:331u2013335.

2. Yangyang Shao, Ning Lu, Chen Cai, Fan Zhou, Shanshan Wang, Zhihu Zhao*, Guoping Zhao*, Jinqiu Zhou*, Xiaoli Xue* and Zhongjun Qin* (2018). A single circular chromosome yeast. Cell Research. 10.1038/s41422-018-0110-y.

3. Yangyang Shao, Ning Lu, Zhongjun Qin* and Xiaoli Xue* (2018). CRISPR-Cas9 facilitated multiple-chromosome fusion in Saccharomyces cerevisiae. ACS Synthetic Biology. 10.1021/acssynbio.8b00397.

4. Yangyang Shao, Ning Lu, Xiaoli Xue* and Zhongjun Qin* (2018). CRISPR-Cas9 facilitated multiple-chromosome fusion in Saccharomyces cerevisiae. Nature Protocol. Invited.

5. Jianting Zhou, Ronghai Wu, Xiaoli Xue* and Zhongjun Qin* (2016) CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA. Nucleic Acids Research. 4(14):e124.

6. Xiaoli Xue*, Tao Wang, Peng Jiang, Yangyang Shao, Min Zhou, Li Zhong, Ronghai Wu, Jianting Zhou, Haiyang Xia, Guoping Zhao and Zhongjun Qin* (2015) MEGA (Multiple Essential Genes Assembling) deletion and replacement method for genome reduction in Escherichia coli. ACS Synthetic Biology. 4(6):700u2013706.

7. Jian Chen, Haiyang Xia*, Fujun Dang, Qingyu Xu, Wenjun Li and Zhongjun Qin* (2015) Characterization of the chromosomal integration of Saccharopolyspora plasmid pCM32 and its application to improve production of spinosyn in Saccharopolyspora spinosa. Applied Microbiology and Biotechnology. 99:10141u201310149.

8. Yalan Chen, Huarong Tan and Zhongjun Qin* (2013) Characterization of a replication locus and formation of a higher-order complex between RepA protein and two inverted-repeats in Streptomyces plasmid pSV1. FEMS Microbiology Letters. 349: 144u2013152.

9. Shiyuan Peng, Ana Zeng, Li Zhong, Ran Zhang, Min Zhou, Qiuxiang Cheng, Liqian Zhao, Tao Wang, Huarong Tan and Zhongjun Qin* (2013) Three functional replication origins of the linear and artificially-circularized plasmid SCP1 of Streptomyces coelicolor. Microbiology. 159: 2127u20132140.

10. Ran Zhang, Haiyang Xia, Qingyu Xu, Fujun Dang and Zhongjun Qin* (2013) Recombinational cloning of the antibiotic biosynthetic gene clusters in linear plasmid SCP1 of Streptomyces coelicolor A3(2). FEMS Microbiology Letters. 345: 39u201348.

11. Pengfei Xie, Ana Zeng and Zhongjun Qin* (2013) A putative transglycosylase encoded by SCO4132 influences morphological differentiation and actinorhodin production in Streptomyces coelicolor. Acta Biochimica et Biophysica Sinica. 45(4):296u2013302.

12. Tao Wang, Zhenhua Chen, Qiuxiang Cheng, Min Zhou, Xinli Tian, Pengfei Xie, Li Zhong, Meijuan Shen and Zhongjun Qin* (2012) Characterization of replication and conjugation of plasmid pWTY27 from a widely distributed Streptomyces species. BMC Microbiology. 12:253.

13. Zhenhua Chen, Li Zhong, Meijuan Shen, Ping Fang and Zhongjun Qin* (2012) Characterization of Streptomyces plasmid-phage pFP4 and its evolutionary implications. Plasmid. 68:170u2013178.

14. Min Zhou, Xinyun Jing, Pengfei Xie, Weihua Chen, Tao Wang, Haiyang Xia and Zhongjun Qin* (2012) Sequential deletion of all the PKS and NRPS biosynthetic gene clusters and a 900-kb subtelomeric sequence of the linear chromosome of Streptomyces coelicolor. FEMS Microbiology Letters. 333(2):169u2013179.

15. Yun Fan, Yumei Dai, Qiuxiang Cheng, Guangjun Zhang, Dongshu Zhang, Ping Fang*, Hang Wu, Linquan Bai*, Zixin Deng and Zhongjun Qin* (2012) A self-ligation method for PCR-sequencing the telomeres of Streptomyces and Mycobacterium linear replicons. Journal of Microbiological Methods. 90:105u2013107.

16. Weihua Chen and Zhongjun Qin* (2011) Development of a gene cloning system in a fast-growing and moderately thermophilic Streptomyces species and heterologous expression of Streptomyces antibiotic biosynthetic gene clusters. BMC Microbiology. 11(1):243.

17. Ana Zeng, Tao Wang, Haiyang Xia, Shiyuan Peng, Weihua Chen, Chenglin Jiang, Lihua Xu, Li Zhong, Meijuan Shen and Zhongjun Qin* (2011) Development of a vector and host system and characterization of replication of plasmid pSQ10 in moderately halophilic Nocardiopsis. Acta Biochimica et Biophysica Sinica. 43(9):738u2013743.

18. Peng Guo, Qiuxiang Cheng, Pengfei Xie, Yun Fan, Weihong Jiang and Zhongjun Qin* (2011) Characterization of the multiple CRISPR loci on Streptomyces linear plasmid pSHK1. Acta Biochimica et Biophysica Sinica. 43(8):630u2013639.

19. Li Zhong, Qiuxiang Cheng, Xinli Tian, Liqian Zhao and Zhongjun Qin* (2010) Characterization of the replication, transfer and plasmid/lytic phage cycle of the Streptomyces plasmid-phage pZL12. Journal of Bacteriology. 192:3747u20133754.

20. Ran Zhang, Shiyuan Peng and Zhongjun Qin* (2010) Two internal origins of replication on Streptomyces linear plasmid pFRL1. Applied and Environmental Microbiology. 76(17):5676u20135683.

21. Zhiqun Lu, Pengfei Xie and Zhongjun Qin* (2010) Promotion of markerless deletion of the actinorhodin biosynthetic gene cluster in Streptomyces coelicolor. Acta Biochimica et Biophysica Sinica. 42(10):717u2013721.

22. Pengfei Xie, Ana Zeng and Zhongjun Qin* (2009) cmdABCDEF, a cluster of genes encoding membrane proteins for differentiation and antibiotic production in Streptoymyces coelicolor A3(2). BMC Microbiology. 9:157.

23. Yongqiang Tian, Weihong Jiang, Guoping Zhao* and Zhongjun Qin* (2009) In vivo conjugation-coupled recombinational cloning of a Streptomyces lividans chromosomal telomeric DNA using a linear plasmid. Biotechnology Letter. 31(8):1253u20131258.

24. Ran Zhang, Haiyang Xia, Peng Guo and Zhongjun Qin* (2009) Variation in the replication loci of Streptomyces linear plasmids. FEMS Microbiology Letters. 290:209u2013216.

25. Ran Zhang, Ana Zeng, Ping Fang and Zhongjun Qin* (2008) Characterization of the replication and conjugation loci of Streptomyces circular plasmids pFP11 and pFP1 and their ability to propagate in linear mode with artificially attached telomeres. Applied and Environmental Microbiology. 74(11):3368u20133376.

26. Jingyi Zhao, Li Zhong, Meijuan Shen, Zhijie Xia, Qiuxiang Cheng, Xia Sun, Guoping Zhao, Yuezhong Li* and Zhongjun Qin* (2008) Discovery of an autonomously-replicating plasmid pMF1 from Myxococcus fulvus and the development of a gene cloning system in Myxococcus Xanthus. Applied and Environmental Microbiology. 74(7):1980u20131987.

27. Mingxuan Xu, Yingmin Zhu, Ran Zhang, Meijuan Shen, Weihong Jiang, Guoping Zhao* and Zhongjun Qin* (2006) Characterization of the genetic components of Streptomyces lividans linear plasmid SLP2 for replication in circular and linear modes. Journal of Bacteriology.188:6851u20136857.

28. Ran Zhang, YongYang, Ping Fang, Chenglin Jiang, Lihua Xu, Yingmin Zhu, Meijuan Shen, Haiyang Xia, Jianfu Zhao, Tongbing Chen and Zhongjun Qin* (2006) Diversity of telomere palindromic sequences and replication genes among Streptomyces linear plasmids. Applied and Environmental Microbiology. 72(9):5728u20135733.

29. Meijuan Shen, Ping Fang, Deqiang Xu, Yalei Zhang, Weihuan Cao, Yingmin Zhu, JianfuZhao and Zhongjun Qin* (2006) Replication and Inheritance of Nocardia Plasmid pC1. FEMS Microbiology Letters. 261:47u201352.

30. Haiyang Xia, Yongqiang Tian, Ran Zhang, Kaichun Lin and Zhongjun Qin* (2006) Characterization of Nocardia Plasmid pXT107. Acta Biochimica et Biophysica Sinica. 38(9):620u2013624.

31. Mingxuan Xu, Yingmin Zhu, Meijuan Shen, Weihong Jiang, Guoping Zhao* and Zhongjun Qin* (2006) Characterization of the essential genetic components for conjugal transfer of Streptomyces lividans linear plasmid SLP2. Progress in Biochemistry and Biophysics. 33(10):1u20138.

32. Yongqiang Tian, Pei Hao, G.uoping Zhao* and Zhongjun Qin* (2005) Cloning and characterization of the chromosomal replication origin region of Amycolatopsis mediterranei U32. Biochemical and Biophysical Research Communications. 333(1):14-20.

33. Zhongjun Qin, Meijuan Shen and Stanley N. Cohen* (2003) Identification and characterization of a pSLA2 plasmids locus required for linear DNA replication and circular plasmids stable inheritance in Streptomyces lividans. Journal of Bacteriology. 185 (22):6575u20136582.

34. Zhongjun Qin and Stanley N. Cohen* (2002) Survival mechanisms for Streptomyces linear replicons after telomere damage. Molecular Microbiology. 45(3):785u2013794.

35. Zhongjun Qin and Stanley N. Cohen* (2000) Long palindrome formed in Streptomyces by nonrecombinational intra-strand annealing. Genes&Development. 14:1789u20131796.

36. Zhongjun Qin and Stanley N. Cohen* (1998) Replication at the telomeres of Streptomyces linear plasmid pSLA2. Molecular Microbiology. 28:893u2013903.

37. Zhongjun Qin, Kaiman Peng, Xiufen Zhou, Rongfang Liang, Qi Zhou, Huakui Chen, David A. Hopwood, Tobias Kieser and Zixin Deng* (1994) Development of a gene cloning system for Streptomyces hygroscopicus subsp. yingchenggenesis, a producer of the three useful antifungal compounds, by elimination of three barriers to DNA transfer. Journal of Bacteriology. 176:2090u20132095.